Evaluation of Fibrinolytic Capacity in Plasma during Thrombolytic Therapy with Single (scu-PA) or Two-chain Urokinase Type Plasminogen Activator (tcu-PA) by a Combined Assay System for Urokinase Type Plasminogen Activator Antigen and Function

Johann Wojta; Bernd R Binder; Kurt Huber; Richard L Hoover

doi:10.1055/s-0038-1646578

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1989; 61(02): 289-293
DOI: 10.1055/s-0038-1646578

Original Article

Schattauer GmbH Stuttgart

Evaluation of Fibrinolytic Capacity in Plasma during Thrombolytic Therapy with Single (scu-PA) or Two-chain Urokinase Type Plasminogen Activator (tcu-PA) by a Combined Assay System for Urokinase Type Plasminogen Activator Antigen and Function

Johann Wojta

¹The Department of Pathology and Pediatrics, Vanderbilt University, Nashville, TN, USA

²The Laboratory for Clinical Experimental Physiology, Department of Medical Physiology, Universityof Vienna, Austria

,

Bernd R Binder

²The Laboratory for Clinical Experimental Physiology, Department of Medical Physiology, Universityof Vienna, Austria

,

Kurt Huber

³The Department of Cardiology, University of Vienna, Austria

,

Richard L Hoover

¹The Department of Pathology and Pediatrics, Vanderbilt University, Nashville, TN, USA

› Author Affiliations

Abstract

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Summary

A combined assay for urokinase type plasminogen activator (u-PA) activity and antigen determination in plasma samples is described. This assay is based on binding of u-PA to an antibody immobilized on a microtiter plate followed by determination of the enzymatic activity of the bound u-PA. Thereafter bound u-PA antigen can be quantified by means of a specific peroxidase labelled monoclonal antibody against u-PA. By use of this assay system u-PA activity and antigen can be determined with lower detection limits of 0.08 IU/ml and 1.0 ng/ml, respectively, and intraassay as well as interassay coefficients of variation of 10% and 12% for activity and 5% and 7% for antigen determinations, respectively. Normal plasma levels of u-PA antigen could be determined to be 1.88 nglml ± 0.61. Furthennore, this assay system allows specific quantification of u-PA antigen and activity during thrombolytic therapy.

Keywords

u-PA ELISA - u-PA activity - Monoclonal antibodies - scu-PA - Thrombolytic therapy monitoring

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References

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